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Catalog Number | orb606680 |
---|---|
Category | Antibodies |
Description | This MAb reacts with MUC1, a large transmembrane glycoprotein expressed on the ductal surface of normal glandular epithelia. It is used as tracer agent in CA15.3 assays. The extracellular domain of MUC1 largely consists of a highly conserved, O-glycosylated 20 amino acids tandem repeat which can occur 30-100 times per molecule depending on the length of the allele involved. In the vast majority of human carcinomas this protein is up-regulated and poorly glycosylated and appears on the cell surface in a non-polarized fashion. The dominant epitope of this MAb involves both amino acids as well as sugar moieties. Its epitope is destroyed by desialylation i.e. treatment with Neuraminidase. |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | 115D8 |
Tested applications | IHC-P |
Reactivity | Human |
Isotype | Mouse IgG2b, kappa |
Immunogen | Human milk fat globule membranes were used as the immunogen for this MUC1 antibody. |
Dilution range | Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT |
Purity | Protein G affinity chromatography |
Conjugation | Unconjugated |
Formula | 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide |
Hazard Information | This MUC1 antibody is available for research use only. |
UniProt ID | P15941 |
Storage | Store the MUC1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide). |
Buffer/Preservatives | 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide |
Note | For research use only |
Application notes | The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the MUC1 antibody to be titered up or down for optimal performance.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. |
Expiration Date | 12 months from date of receipt. |
IHC testing of FFPE human stomach with MUC1 antibody (clone 115D8). HIER: requires steaming of sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min.
IHC testing of FFPE human breast carcinoma with MUC1 antibody (clone 115D8). HIER: requires steaming of sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min.
FACS, IF, IHC-P, WB | |
Human | |
Mouse | |
Monoclonal | |
Unconjugated |
ELISA, IHC-P, WB | |
Human | |
Rabbit | |
Recombinant | |
Unconjugated |
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