MUC1 Antibody / Mucin-1

• Catalog Number: orb606520
• Category: Antibodies
• Description: Mucin-1/MUC1 protein is encoded by the MUC1 gene in humans. This gene encodes a membrane-bound protein that is a member of the mucin family. Family members are O-glycosylated proteins that play an essential role in forming protective mucous barriers on epithelial surfaces. MUC1 is a transmembrane protein normally expressed on the apical borders of secretory epithelial cells. Overexpression is often associated with colon, breast, ovarian, lung and pancreatic cancers. The dominant epitope of this MAb involves both amino acids as well as sugar moieties. Its epitope is destroyed by desialylation i.e. treatment with Neuraminidase.
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: MUC1/1887R
• Tested applications: IHC-P, WB
• Reactivity: Human
• Isotype: Rabbit IgG, kappa
• Immunogen: Human milk fat globule membranes were used as the immunogen for this recombinant Mucin-1 antibody.
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT,Western blot: 1-2ug/ml
• Purity: Protein A affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This recombinant Mucin-1 antibody is available for research use only.
• Entrez: 4582
• UniProt ID: P15941
• Storage: Store the recombinant Mucin-1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the recombinant Mucin-1 antibody to be titered up or down for optimal performance.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

Western blot testing of human MCF7 cell lysate with recombinant Mucin-1 antibody (clone MUC1/1887R). This glycoprotein is commonly visualized between 120~500 kDa.

SDS-PAGE analysis of purified, BSA-free recombinant Mucin-1 antibody (clone MUC1/1887R) as confirmation of integrity and purity.

IHC testing of FFPE human breast carcinoma with recombinant Mucin-1 antibody (clone MUC1/1887R). Required HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.