MUC1 Antibody / Mucin-1

• Catalog Number: orb606501
• Category: Antibodies
• Description: This MAb reacts with MUC1, a large transmembrane glycoprotein expressed on the ductal surface of normal glandular epithelia. It is used as tracer agent in CA15.3 assays. The extracellular domain of MUC1 largely consists of a highly conserved, O-glycosylated 20 amino acids tandem repeat which can occur 30-100 times per molecule depending on the length of the allele involved. In the vast majority of human carcinomas this protein is up-regulated and poorly glycosylated and appears on the cell surface in a non-polarized fashion. The dominant epitope of this MAb involves both amino acids as well as sugar moieties. Its epitope is destroyed by desialylation i.e. treatment with Neuraminidase.
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: MUC1/2278R
• Tested applications: IHC-P
• Reactivity: Human
• Isotype: Rabbit IgG, kappa
• Immunogen: Human milk fat globule membranes were used as the immunogen for this recombinant Mucin-1 antibody.
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Purity: Protein A affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This recombinant Mucin-1 antibody is available for research use only.
• Entrez: 4582
• UniProt ID: P15941
• Storage: Store the recombinant Mucin-1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Titration of the recombinant Mucin-1 may be required due to differences in protocols and secondary/substrate sensitivity.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

IHC testing of FFPE human breast carcinoma with recombinant Mucin-1 antibody (clone MUC1/2278R). Required HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.

SDS-PAGE analysis of purified, BSA-free recombinant Mucin-1 antibody (clone MUC1/2278R) as confirmation of integrity and purity.