mtTFA TFAM Antibody (monoclonal, 4D9)

• Catalog Number: orb610917
• Category: Antibodies
• Description: mtTFA TFAM Antibody (monoclonal, 4D9)
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 4D9
• Tested applications: FC, IHC, WB
• Reactivity: Human
• Isotype: Mouse IgG2b
• Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human mtTFA (214-241aa EMKSWEEQMIEVGRKDLLRRTIKKQRKY), different from the related mouse and rat sequences by five amino acids.
• Concentration: 0
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 40 kDa
• UniProt ID: Q00059
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Transcription factor A, mitochondrial; mtTFA; Mito
• Note: For research use only
• Application notes: Add 0.2ml of distilled water will yield a concentration of 500μg/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of CACO-2 cells using anti-FAM antibody(Blue line).Isotype control antibody (Green line) was mouse IgG.Unlabelled sample (Red line) was also used as a control.

IHC analysis of TFAM using anti-TFAM antibody.

IHC analysis of TFAM using anti-TFAM antibody.

WB analysis using anti ZO-1 antibody.Lane 1:HEK293 tissue, Lane 2:K562 cell, Lane 3:Caco-2 cell, Lane 4:Raji cell, Lane 5:A549 cell