MTOR Antibody

• Catalog Number: orb1253736
• Category: Antibodies
• Description: MTOR Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, WB
• Reactivity: Human
• Immunogen: mTOR (Phospho-Ser2448) antibody was raised against a peptide sequence around phosphorylation site of serine 2448 (T-D-S (p) -Y-S) derived from Human mTOR.
• Concentration: 1 mg/mL
• Dilution range: Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 289 kDa
• Target: MTOR
• UniProt ID: P42345
• NCBI: NP_004949.1
• Storage: Store antibody at -20°C for up to one year.
• Buffer/Preservatives: Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Alternative names: FRAP, FRAP1, FRAP2, RAFT1, RAPT1, HSP27, HSP28, FR
• Note: For research use only
• Application notes: Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200
• Expiration Date: 12 months from date of receipt.

Western blot analysis of lysed extracts from 293 cells untreated or treated with PMA using mTOR (Phospho-Ser2448).

Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using mTOR (Phospho-Ser2448). Bottom Image: Immunofluorescence staining of methanol-fixed MCF cells using mTOR (Phospho-Ser2448).