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Catalog Number | orb1928003 |
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Category | Antibodies |
Description | Affinity Purified Rabbit Polyclonal Antibody (Pab) |
Species/Host | Rabbit |
Clonality | Polyclonal |
Clone Number | RB23653 |
Tested applications | FC, WB |
Reactivity | Human |
Isotype | Rabbit IgG |
Dilution range | WB: 1:500, WB: 1:1000, FC: 1:10~50 |
Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
Conjugation | Unconjugated |
MW | 39375 Da |
Target | This MTNR1A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 209-239 amino acids from the Central region of human MTNR1A. |
UniProt ID | P48039 |
NCBI | NP_005949.1 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
Alternative names | Melatonin receptor type 1A, Mel-1A-R, Mel1a recept Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of MTNR1A Antibody (Center) in CEM cell line lysates (35 ug/lane). MTNR1A (arrow) was detected using the purified Pab.
MTNR1A Antibody (Center) flow cytometric analysis of CEM cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
All lanes: Anti-MTNR1A Antibody (Center) at 1:500 dilution. Lane 1: HepG2 whole cell lysate. Lane 2: CCRF-CEM whole cell lysate. Lane 3: Hela whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 36 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.