MTAP Antibody

• Catalog Number: orb606606
• Category: Antibodies
• Description: Recognizes a protein of 31kDa, which is identified as MTAP (5'-deoxy-5'-methylthioadenosine phosphorylase). It catalyzes the reversible phosphorolysis of methylthioadenosine, which is important in polyamine metabolism and for the salvage of adenine and methionine. The gene encoding MTAP is linked to the tumor suppressor gene, p16INK4A. Deficient levels of MTAP can occur in cancers primarily through co-deletion of the MTAP gene and the p16INK4A gene. Cells expressing MTAP and possessing adenine salvage pathway activity may be less susceptible to malignancy due to growth-inhibitory actions of agents (e.g. antifolates), whose mechanism of action, in part, involves this de novo purine pathway.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: MTAP/1813
• Tested applications: ELISA, IHC-P, WB
• Reactivity: Human
• Isotype: Mouse IgG2b, kappa
• Immunogen: A portion of amino acids 97-196 from the human protein was used as the immunogen for the MTAP antibody.
• Dilution range: ELISA: 2-4ug/ml (order BSA/azide-free format),Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This MTAP antibody is available for research use only.
• UniProt ID: Q13126
• Storage: Store the MTAP antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the MTAP antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

Western blot testing of human A431 cell lysate with MTAP antibody (clone MTAP/1813). Expected molecular weight: 26-38 kDa (multiple isoforms).

IHC staining of FFPE human kidney with MTAP antibody (clone MTAP/1813). Required HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

Analysis of HuProt (TM) microarray containing more than 19, 000 full-length human proteins using MTAP antibody (clone MTAP/1813). These results demonstrate the foremost specificity of the MTAP/1813 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

SDS-PAGE analysis of purified, BSA-free MTAP antibody (clone MTAP/1813) as confirmation of integrity and purity.

Western blot testing of human 1) HeLa, 2) A431, 3) HepG2 and 4) HAP-1 cell lysate with MTAP antibody (clone MTAP/1813). Expected molecular weight: 26-38 kDa (multiple isoforms).