MSX1 Antibody

• Catalog Number: orb1247248
• Category: Antibodies
• Description: MSX1 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Predicted Reactivity: Bovine, Mouse, Porcine, Rat
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: TSLPLGVKVEDS-C
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Preliminary experiments gave an approx. 55-60kDa band in Human Muscle and Heart lysates at 1ug/ml. Further, this band was successfully blocked by incubation with the immunising peptide. Please note that currently we cannot find an explanation in the literature for the band we observe given the predicted size of approx. 31.4kDa according to Human NP_002439.2. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?Immunohistochemistry:In paraffin embedded Human Prostate shows staining of nucleoli in a few epithelial cells. Recommended concentration, 2-4ug/ml. Data obtained from a previous batch (different goat).
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: MSX1
• UniProt ID: P28360
• NCBI: NP_002439.2
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: MSX1, HOX7, HYD1, msh homeo box homolog 1 (Drosoph
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Preliminary experiments gave an approx. 55-60kDa band in Human Muscle and Heart lysates at 1ug/ml. Further, this band was successfully blocked by incubation with the immunising peptide. Please note that currently we cannot find an explanation in the literature for the band we observe given the predicted size of approx. 31.4kDa according to Human NP_002439.2. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?Immunohistochemistry:In paraffin embedded Human Prostate shows staining of nucleoli in a few epithelial cells. Recommended concentration, 2-4ug/ml. Data obtained from a previous batch (different goat).
• Expiration Date: 12 months from date of receipt.

orb1247248 (2.5 ug/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

orb1247248 (0.3 ug/ml) staining of Human Prostate lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.