MSRA Antibody (N-term)

• Catalog Number: orb1429026
• Category: Antibodies
• Description: Rabbit polyclonal antibody to MSRA.
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB18651
• Tested applications: FC, IF, IHC-P, WB
• Reactivity: Human, Mouse
• Isotype: Rabbit IgG
• Immunogen: Synthetic Peptide
• Dilution range: IF: 1:100, WB: 1:1000, WB: 1:1000, IHC-P: 1:10~50, FC: 1:10~50
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Conjugation: Unconjugated
• MW: 26132
• Target: MSRA
• UniProt ID: Q9UJ68
• NCBI: NP_001129142.1, NP_001129143.1, NP_001186658.1, NP_036463.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Mitochondrial peptide methionine sulfoxide reducta
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Anti-MSRA Antibody (N-term) at 1:1000 dilution + human kidney lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 26 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with MSRA Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Immunofluorescent analysis of A549 cells, using MSRA Antibody (N-term). Diluted at 1:100 dilution. Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Dylight Fluor 554 (red) conjugated Phalloidin (red).

MSRA Antibody (N-term) flow cytometric analysis of MDA-MB435 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Western blot analysis of MSRA antibody (N-term) in mouse kidney tissue lysates (35 ug/lane). MSRA (arrow) was detected using the purified Pab.