MSLN Antibody

• Catalog Number: orb1671490
• Category: Antibodies
• Description: MSLN Antibody
• Clonality: Recombinant
• Clone Number: 11-25
• Tested applications: ELISA, FC, IP
• Reactivity: Human
• Isotype: IgG kappa
• Immunogen: The original antibody was generated by intraperitoneally immunizing BALB/c mice with the purified recombinant mesothelin protein.
• Concentration: batch dependent
• Conjugation: Unconjugated
• Target: MSLN
• UniProt ID: Q13421
• Storage: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: MSLN, CAK1 antigen, Pre-pro-megakaryocyte-potentia
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunofluorescence staining of HeLa cells with anti-Mesothelin 11-25 Immunofluorescence analysis of paraformaldehyde fixed HeLa cells stained with the chimeric r version of 11-25 (orb1671490) (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution)- showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom orb1671490- DAPI- merged channels and an isotype control. The isotype control was an unknown specificity antibody (3.0) followed by staining with Alexa Fluor® 488 secondary antibody.

Flow cytometry using the Anti-Mesothelin antibody 11-25 (Paraformaldehyde fixed HeLa cells permewith 0.5% Triton were stained with anti-unknown specificity antibody (3.0; isotype control - black line) or the r version of 11-25 (orb1671490 - blue line) at a dilution of 1:100 for 1h at RT. After washing- the bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.