MSH6 Antibody

• Catalog Number: orb607141
• Category: Antibodies
• Description: The finding that mutations in DNA mismatch repair genes are associated with hereditary nonpolyposis colorectal cancer (HNPCC) has resulted in considerable interest in the understanding of the mechanism of DNA mismatch repair. Initially, inherited mutations in the MSH2 and MLH1 homologs of the bacterial DNA mismatch repair genes mutS and mutL were demonstrated at high frequency in HNPCC and were shown to be associated with microsatellite instability. A member of the mismatch repair family, GTBP (also designated MSH6), is an MSH2-related protein that binds to DNA containing G/T mismatches. Findings suggest that the mismatch-binding factor in human cells is composed of a heterodimer of GTBP and MSH2.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: MSH6/3086
• Tested applications: ELISA, FACS, IF, IHC-P, WB
• Reactivity: Human
• Isotype: Mouse IgG2b, kappa
• Immunogen: A recombinant human MSH6 protein fragment within amino acids 374-540 was used as the immunogen for the MSH6 antibody.
• Dilution range: ELISA (order BSA/sodium azide-free format for coating),Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This MSH6 antibody is available for research use only.
• UniProt ID: P52701
• Storage: Store the MSH6 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the MSH6 antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
• Expiration Date: 12 months from date of receipt.

Immunofluorescence staining of PFA-fixed human MCF-7 cells with MSH6 antibody (green, clone MSH6/3086) and Phalloidin (red).

IHC staining of FFPE human small intestine with MSH6 antibody (clone MSH6/3086). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.

IHC staining of FFPE human colon carcinoma with MSH6 antibody (clone MSH6/3086). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.

Western blot testing of human HCT116 lysate with MSH6 antibody (clone MSH6/3086). Predicted molecular weight ~160 kDa.

Flow cytometry testing of fixed and permeabilized human MCF-7 cells with MSH6 antibody (clone MSH6/3086); Red=isotype control, Blue= MSH6 antibody.

Analysis of HuProt (TM) microarray containing more than 19000 full-length human proteins using MSH6 antibody (clone MSH6/3086). These results demonstrate the foremost specificity of the MSH6/3086 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

SDS-PAGE analysis of purified, BSA-free MSH6 antibody (clone MSH6/3086) as confirmation of integrity and purity.