MSH6 antibody

• Catalog Number: orb1151659
• Category: Antibodies
• Description: Rabbit monoclonal antibody to MSH6
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: MSH6/6654R
• Tested applications: ELISA, IHC-P, WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Immunogen: A portion of amino acids 1-100 was used as the immunogen for the recombinant MSH6 antibody.
• Dilution range: ELISA (order BSA-free format for coating),Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
• Purity: Protein A/G affinity
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This recombinant MSH6 antibody is available for research use only.
• UniProt ID: P52701
• Storage: Aliquot the recombinant MSH6 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the recombinant MSH6 antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of human HCT-116 cell lysate using recombinant MSH6 antibody (clone MSH6/6654R). Expected molecular weight: 120-160 kDa depending on phosphorylation level.

IHC staining of FFPE human colon carcinoma tissue with recombinant MSH6 antibody (clone MSH6/6654R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human colon tissue from a patient with Lynch syndrome using recombinant MSH6 antibody (clone MSH6/6654R). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.