MSH2 antibody

• Catalog Number: orb1151551
• Category: Antibodies
• Description: Mouse monoclonal antibody to MSH2
• Species/Host: Mouse
• Clonality: Recombinant
• Clone Number: rMSH2/6548
• Tested applications: IHC-P, WB
• Reactivity: Canine, Feline, Human
• Isotype: Mouse IgG2a, kappa
• Immunogen: Recombinant full-length human MSH2 protein was used as the immunogen for the recombinant MSH2 antibody.
• Dilution range: Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
• Purity: Protein A/G affinity
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This recombinant MSH2 antibody is available for research use only.
• UniProt ID: P43246
• Storage: Aliquot the recombinant MSH2 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the recombinant MSH2 antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

IHC staining of FFPE human colon tissue with recombinant MSH2 antibody (clone rMSH2/6548). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human colon tissue with recombinant MSH2 antibody (clone rMSH2/6548). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE cat lymph node tissue with recombinant MSH2 antibody (clone rMSH2/6548). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE dog lymph node tissue with recombinant MSH2 antibody (clone rMSH2/6548). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Western blot testing of human HCT-116 cell lysate using recombinant MSH2 antibody (clone rMSH2/6548). Expected molecular weight ~105 kDa.

SDS-PAGE analysis of purified, BSA-free recombinant MSH2 antibody (clone rMSH2/6548) as confirmation of integrity and purity.