Ms4a1 Antibody

• Catalog Number: orb1671745
• Category: Antibodies
• Description: Ms4a1 Antibody
• Clonality: Recombinant
• Clone Number: 18B12
• Tested applications: Depletion, FA, FC, IF, WB
• Reactivity: Mouse
• Isotype: IgG kappa
• Immunogen: This antibody was generated by immunizing CD20−/− mice with CD20-transfected cell lines (300.18 and 70Z3) and CD20-peptide-keyhole limpet hemocyanin conjugate.
• Concentration: batch dependent
• Conjugation: Unconjugated
• Target: Ms4a1
• UniProt ID: P19437
• Storage: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: Ly-44, B-cell differentiation antigen Ly-44, B-lym
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow-cytometry using the Anti-CD20 antibody 18B12. Splenocytes were stained with anti-Fluorescein IgG antibody (4-4-20; isotype control - black line) or the r version of 18B12 (orb1671745 - blue line) at a dilution of 1:100 for 1h at RT. After washing- bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Immunofluorescence staining of U937 cells using anti-CD20 antibody (18B12). Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes immobilized on Shi-fix™ cover-slips and stained with the chimeric r version of 18B12 (orb1671745) at 10 ug/ml followed by Alexa Fluor® 488 secondary antibody (2 ug/ml)- showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right- top-bottom orb1671745- DAPI- merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody (3.0) followed by Alexa Fluor® 488 secondary antibody.