MRPL12 Antibody

• Catalog Number: orb1255912
• Category: Antibodies
• Description: MRPL12 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, WB
• Reactivity: Human
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-198 of human MRPL12 (NP_002940.2).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IHC: 1:50 - 1:100
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 21kDa
• Target: MRPL12
• UniProt ID: P52815
• Storage: Store at -20˚°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: Liquid
• Alternative names: 5c5-2, L12mt, MRP-L31/34, MRPL7, MRPL7/L12, RPML12
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:100
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using MRPL12 antibody (orb1255912) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.

Immunohistochemistry of paraffin-embedded human esophageal smooth muscle using MRPL12 antibody (orb1255912) at dilution of 1:100 (40x lens).