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Catalog Number | orb1146973 |
---|---|
Category | Proteins |
Description | Mouse TNFR2, His Tag is expressed from human 293 cells (HEK293). It contains AA Val 23 - Gly 258 (Accession # P25119-1). |
Reactivity | Mouse |
Tag | C-10×His |
Form/Appearance | Powder |
Purity | 90% |
Conjugation | Unconjugated |
MW | 27.2 kDa |
Target | TNFR2, CD120b, TNFRSF1B |
UniProt ID | P25119 |
Protein Sequence | NP_035740.2 |
Source | Mouse TNFR2, His Tag (orb1146973) is expressed from human 293 cells (HEK293). It contains AA Val 23 - Gly 258 (Accession # P25119-1). |
Expression System | HEK293 |
Biological Origin | Mouse |
Expression Region | Val 23 - Gly 258 |
Endotoxins | 1.0 EU per μg |
NCBI | NP_035740.2 |
Storage | -20℃ |
Buffer/Preservatives | PBS, pH7.4 |
Alternative names | TNFRSF1B,CD120b,TBPII,TNF-R-II,TNF-R75,TNFBR,TNFR1 Read more... |
Note | For research use only |
Application notes | This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 27.2 kDa. The protein migrates as 45-55 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Expiration Date | 6 months from date of receipt. |
The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 26.2 kDa after removal of the signal peptide. The apparent molecular mass of mTNFRSF1B-His is approximately 25-55 kDa due to glycosylation. | |
Mammalian |
> 97%, determined by SDS-PAGE | |
This protein contains the extracellular domain of mouse TNFRSF1B (NP_035740.2) (Met 1-Gly 258) was fused with a polyhistidine tag at the C-terminus and expressed from HEK293 Cells. |
SDS-PAGE: 97.9%; SEC-HPLC: 99.6% | |
26.8 kDa (predicted); 45 kDa (reducing condition, due to glycosylation) |
> 95 % by SDS-PAGE. | |
Recombinant Mouse TNFR2 / TNFRSF1B Protein is produced by HEK293 Cells expression system. The target protein is expressed with sequence (Met1-Gly258) of mouse TNFR2 / TNFRSF1B (Accession #NP_035740.2) fused with an Fc tag at the C-terminus. |