Mouse TNF-alpha Protein, His Tag

• Catalog Number: orb1496307
• Category: Proteins
• Description: Mouse TNF-alpha, His Tag is expressed from human 293 cells (HEK293). It contains AA Leu 80 - Leu 235 (Accession # P06804).
• Reactivity: Mouse
• Tag: C-10×His
• Form/Appearance: Powder
• Purity: 95%
• Conjugation: Unconjugated
• MW: 19.1 kDa
• Target: TNF-alpha
• UniProt ID: P06804
• Source: Mouse TNF-alpha, His Tag (orb1496307) is expressed from human 293 cells (HEK293). It contains AA Leu 80 - Leu 235 (Accession # P06804).
• Expression System: HEK293
• Biological Origin: Mouse
• Biological Activity: Immobilized Mouse TNF-alpha, His Tag at 0.2 μg/mL (100 μL/well) can bind Human TNFR1, Fc Tag with a linear range of 0.1-1 ng/mL (QC tested).
• Expression Region: Leu 80 - Leu 235
• Endotoxins: 1.0 EU per μg
• Storage: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
• Buffer/Preservatives: PBS, pH7.4
• Alternative names: DIF,TNF-alpha,TNFA,TNFSF2,cachexin,cachectin,TNFα
• Note: For research use only
• Application notes: This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 19.1 kDa. The protein migrates as 19-20 kDa and 23-24 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expiration Date: 6 months from date of receipt.

Immobilized Mouse TNF-alpha, His Tag (Cat. No. orb1496307) at 0.2 μg/mL (100 μL/well) can bind Human TNFR1, Fc Tag (Cat. No. orb257878) with a linear range of 0.1-1 ng/mL (QC tested).