(Mouse) Tdgf1 Antibody (N-term)

• Catalog Number: orb1926706
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB51515
• Tested applications: FC, WB
• Reactivity: Mouse
• Isotype: Rabbit IgG
• Dilution range: WB: 1:1000, FC: 1:25
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 18655 Da
• Target: This Mouse Tdgf1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 37-70 amino acids from the N-terminal region of mouse Tdgf1.
• UniProt ID: P51865
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Teratocarcinoma-derived growth factor, Cripto grow
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of lysate from mouse F9 cell line, using (Mouse) Tdgf1 Antibody (N-term). Diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20 ug.

Overlay histogram showing F9 cells stained (red line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37°C. The secondary antibody used was Alexa Fluor 488 goat anti-rabbit lgG (H+L) (1583138) at 1/400 dilution for 40 min at 37°C. Isotype control antibody (blue line) was rabbit IgG1 (1 μg/1x10^6 cells) used under the same conditions. Acquisition of > 10000 events was performed.