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| Catalog Number | orb1146825 |
|---|---|
| Category | Assays and Kits |
| Description | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sirtuin 2(SIRT2). Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Sirtuin 2(SIRT2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Sirtuin 2(SIRT2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Sirtuin 2(SIRT2) in the samples is then determined by comparing the OD of the samples to the standard curve. |
| Reactivity | Mouse |
| Range | 1.57-100 ng/mL |
| Assay Time | 3.5h |
| Concentration | 100 ng/mL |
| UniProt ID | Q8VDQ8 |
| Sensitivity | 0.68 ng/mL |
| Alternative names | SIR2L, SIR2-L, SIR2L2, Silent Information Regulato Read more... |
| Note | For research use only |
| Application notes | standard: 100 ng/mL. Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse SIRT2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse SIRT2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse SIRT2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse SIRT2 in the samples is then determined by comparing the OD of the samples to the standard curve |
| Sample Types | tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| Expiration Date | Please enquire. |
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