Mouse Sirt1 Antibody (C-term)

SKU: orb1926867

Description

Mouse Sirt1 Antibody (C-term)

Images & Validation

• Tested Applications: IF, IHC-P, WB
• Dilution range: WB - 1:1000, IHC-P - 1:100-500, IF - 1:25
• Reactivity: Human, Mouse

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Molecular Weight: 80372 Da

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Disclaimer: For research use only

Alternative Names

Sir2l1

Western blot analysis of lysate from mouse F9 cell line, using Mouse Sirt1 Antibody (C-term). Diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20 ug.

Anti-Sirt1 Antibody (C-term) at 1:2000 dilution + MCF-7 whole cell lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 80 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

Immunohistochemical analysis of paraffin-embedded M. brain section using Mouse Sirt1 Antibody (C-term). Diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.

Fluorescent image of A549 cells stained with Mouse Sirt1 Antibody (C-term). Diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).

Quick Database Links

UniProt

Q923E4