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Catalog Number | orb776681 |
---|---|
Category | Assays and Kits |
Description | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Prokineticin 2(PK2) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Prokineticin 2(PK2). |
Reactivity | Mouse |
Range | 312.5-20000 pg/mL |
Concentration | 20000 pg/mL |
UniProt ID | Q9QXU7 |
Sensitivity | 94.4 pg/mL |
Alternative names | PK-2 ELISA Kit, Bv8 ELISA Kit, MIT1 ELISA Kit, KAL Read more... |
Note | For research use only |
Application notes | standard: 20000 pg/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse PK2 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse PK2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse PK2 in the samples is then determined by comparing the OD of the samples to the standard curve |
Sample Types | Serum, plasma and other biological fluids |
Expiration Date | Please enquire. |
Mouse | |
0.68 ng/ml - 500 ng/ml | |
0.68 ng/ml |