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Mouse Noggin Protein

Mouse Noggin Protein

Catalog Number: orb427377

DispatchUsually dispatched within 5-10 working days
$ 250.00
Catalog Numberorb427377
CategoryProteins
DescriptionMouse Noggin proteinÂ
Form/AppearanceSterile Filtered White lyophilized (freeze-dried) powder.
PurityGreater than 95.0% as determined by SDS-PAGE.
Solubility (25°C)It is recommended to be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in 10mM HAc to a concentration of 0.1-1.0 mg/ml. Further dilutions should be made in appropriate buffered solutions.
Protein SequenceMQHYLHIRPAPSDNLPLVDLIEHPDPIFDPKEKDLNETLLRSLLGGHYDPGFMATSPPEDRPGGGGGPAGGAEDLAELDQLLRQRPSGAMPSEIKGLEFSEGLAQGKKQRLSKKLRRKLQMWLWSQTFCPVLYAWNDLGSRFWPRYVKVGSCFSKRSCSVPEGMVCKPSKSVHLTVLRWRCQRRGQRCGWIPIQYPIISECKCSC
SourceEscherichia Coli
Biological ActivityThe ED50 as determined by inhibiting BMP-4-induced alkaline phosphatase production of murine ATDC5 cells is less than 2ng/ml, corresponding to a specific activity of > 5.0 × 105 IU/mg in the presence of 5ng/ml BMP-4.
StorageStability: Lyophilized Mouse Noggin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Mouse Noggin should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles
Buffer/PreservativesLyophilized from a 0.2μm filtered solution in 30% acetonitrile, 0.1% TFA.
Alternative namesNoggin, SYM1, SYNS1, NOG.
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NoteFor research use only
Application notesCytokines And Growth Factors
Expiration Date6 months from date of receipt.
  • Mouse Noggin protein [orb707259]

    ELISA,  MS,  SDS-PAGE,  WB

    Greater than 95% as determined by reducing SDS-PAGE.

    Human cells

    500 μg, 50 μg, 10 μg
  • Noggin/NOG Protein, Mouse, Recombinant (His) [orb1961257]

    > 99.9%

    30 KDa (reducing condition)

    1 mg, 500 μg, 10 μg, 50 μg
  • Noggin/NOG Protein, Mouse, Recombinant (His & Flag) [orb1959990]

    98.00%

    25.16 kDa (predicted). Due to glycosylation, the protein migrates to 28-38 kDa based on Tris-Bis PAGE result.

    1 mg, 500 μg, 100 μg
  • Noggin/NOG Protein, Mouse, Recombinant [orb1959991]

    > 95%

    23.07 kDa (predicted). Due to glycosylation, the protein migrates to 28-33 kDa based on Tris-Bis PAGE result.

    100 μg, 500 μg, 50 μg, 1 mg
  • RecombinantNoggin,Mouse(CHO-expressed) [orb1494673]

    > 95% as analyzed by SDS-PAGE.

    29-31 kDa, observed by reducing SDS-PAGE.

    CHO

    25 μg, 5 μg