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Catalog Number | orb427045 |
---|---|
Category | Tools |
Description | Recombinant of mouse MCP 5 protein |
Form/Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
Purity | Greater than 97.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE. |
Solubility (25°C) | It is recommended to reconstitute the lyophilized MCP5 in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. |
Protein Sequence | GPDAVSTPVT CCYNVVKQKI HVRKLKSYRR ITSSQCPREA VIFRTILDKE ICADPKEKWV KNSINHLDKT SQTFILEPSC LG |
Source | Escherichia Coli |
Biological Activity | Determined by its ability to chemoattract human peripheral blood monocytes using a concentration range of 10.0-50.0 ng/ml. |
Storage | Stability: Lyophilized CCL12 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution MCP5 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles |
Buffer/Preservatives | Lyophilized from a 0.2µm filtered concentrated solution in 1×PBS, pH 7.4. |
Alternative names | C-C motif chemokine 12, MCP-1-related chemokine, M Read more... |
Note | For research use only |
Application notes | Chemokines |
Expiration Date | 12 months from date of receipt. |
> 95% as determined by SDS-PAGE and HPLC. | |
9.3 kDa | |
E.Coli |
Mouse | |
62.5-4000pg/mL | |
37.5 pg/mL |
Mouse | |
62.5-4000pg/mL | |
37.5 pg/mL |
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