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Catalog Number | orb391993 |
---|---|
Category | Proteins |
Description | Recombinant mouse IL6 protein is produced by our E. coli expression system and the target gene encoding Phe25-Thr211 is expressed. |
Tested applications | ELISA, MS, SDS-PAGE, WB |
Reactivity | Mouse |
Form/Appearance | Lyophilized from a 0.2 μM filtered solution of 20mM PB, 150mM NaCl, pH 7.4. |
Purity | Greater than 95% as determined by reducing SDS-PAGE. |
Target | IL6 |
Entrez | 16193 |
UniProt ID | P08505 |
Protein Sequence | MFPTSQVRRG DFTEDTTPNR PVYTTSQVGG LITHVLWEIV EMRKELCNGN SDCMNNDDAL AENNLKLPEI QRNDGCYQTG YNQEICLLKI SSGLLEYHSY LEYMKNNLKD NKKDKARVLQ RDTETLIHIF NQEVKDLHKI VLPTPISNAL LTDKLESQKE WLRTKTIQFI LKSLEEFLKV TLRSTRQT |
Source | E. coli |
Endotoxins | Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test. |
Storage | Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months. |
Buffer/Preservatives | Lyophilized from a 0.2 μM filtered solution of 20mM PB, 150mM NaCl, pH 7.4. |
Alternative names | IFNB2; Interleukin-6; IL-6; B-cell stimulatory fac Read more... |
Note | For research use only |
Application notes | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Expiration Date | 6 months from date of receipt. |
> 97% as determined by SDS-PAGE and HPLC. | |
21.7 kDa | |
E.Coli |
Unconjugated | |
90% | |
23.6 kDa | |
Mouse IL-6, His Tag (orb750344) is expressed from human 293 cells (HEK293). It contains AA Phe 25 - Thr 211 (Accession # P08505-1). |
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 47.9 kDa after removal of the signal peptide. | |
Mammalian |
IF, WB | |
Human | |
Mouse | |
Polyclonal | |
Unconjugated |
IF, IHC-Fr, IHC-P, WB | |
Human | |
Mouse | |
Monoclonal | |
Unconjugated |