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Catalog Number | orb80084 |
---|---|
Category | Proteins |
Description | Interleukin33 Mouse recombinant produced in E.coli is a single, non-glycosylated, polypeptide chain containing 158 amino acids and having a molecular mass of 17.5 kDa. |
Tested applications | FA, HPLC, SDS-PAGE |
Purity | > 97.0% as determined by RP-HPLC and analysis by SDS-PAGE |
Conjugation | Unconjugated |
Target | mIL 33 |
Solubility (25°C) | It is recommended to reconstitute the lyophilized IL-33 in sterile H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions. |
Protein Sequence | SIQGTSLLTQ SPASLSTYND QSVSFVLENG CYVINVDDSG KDQEQDQVLL RYYESPCPAS QSGDGVDGKK LMVNMSPIKD TDIWLHANDK DYSVELQRGD VSPPEQAFFV LHKKSSDFVS FECKNLPGTY IGVKDNQLAL VEEKDESCNN IMFKLSKI. |
Biological Origin | E.coli |
Biological Activity | The ED50 as determined by the dose-dependent stimulation of the proliferation of murine D10S cells is 0.5ng/ml, corresponding to specific activity of 2,000,000 units/mg. |
Storage | Store at 4°C for up to two weeks. For long term storage, aliquot and store at -20°C, avoid freeze/thaw cycles. |
Buffer/Preservatives | The Mouse IL-33 was lyophilized from concentrated (1mg/ml) solution containing 20 mM Phosphate buffer pH-7.4, 150mM sodium chloride, 1mM EDTA and 2mM b-ME. |
Alternative names | Interleukin 33 protein, DVS27 protein, NF-HEV prot Read more... |
Note | For research use only |
Expiration Date | 6 months from date of receipt. |
SDS-PAGE analysis of Mouse IL33 protein
> 98% as determined by SDS-PAGE and HPLC. | |
17.5 kDa | |
E.Coli |
Greater than 95% as determined by SDS-PAGE. | |
17.6 kDa | |
E.coli |
Unconjugated | |
90% | |
19.5 kDa | |
Mouse IL-33, His Tag (orb750372) is expressed from human 293 cells (HEK293). It contains AA Ser 109 - Ile 266 (Accession # Q8BVZ5-1). |
ELISA, MS, SDS-PAGE, WB | |
Greater than 95% as determined by reducing SDS-PAGE. | |
E. coli |