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Catalog Number | orb707228 |
---|---|
Category | Proteins |
Description | Recombinant Mouse IL-1R2 Protein is produced by mammalian expression system and the target gene encoding Phe14-Glu355 is expressed fused with a 6His tag at the C-terminus. |
Tested applications | ELISA, MS, SDS-PAGE, WB |
Reactivity | Mouse |
Form/Appearance | Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. |
Purity | Greater than 95% as determined by reducing SDS-PAGE. |
Target | IL1R2 |
Entrez | 16178 |
UniProt ID | P27931 |
Protein Sequence | FTTPTVVHTG KVSESPITSE KPTVHGDNCQ FRGREFKSEL RLEGEPVVLR CPLAPHSDIS SSSHSFLTWS KLDSSQLIPR DEPRMWVKGN ILWILPAVQQ DSGTYICTFR NASHCEQMSV ELKVFKNTEA SLPHVSYLQI SALSTTGLLV CPDLKEFISS NADGKIQWYK GAILLDKGNK EFLSAGDPTR LLISNTSMDD AGYYRCVMTF TYNGQEYNIT RNIELRVKGT TTEPIPVIIS PLETIPASLG SRLIVPCKVF LGTGTSSNTI VWWLANSTFI SAAYPRGRVT EGLHHQYSEN DENYVEVSLI FDPVTREDLH TDFKCVASNP RSSQSLHTTV KEHHHHHH |
Source | Human cells |
Endotoxins | Endotoxin: Less than 0.1 ng/µg (1 IEU/µg) as determined by LAL test. |
Storage | Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months. |
Buffer/Preservatives | Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. |
Alternative names | IL1RB protein, Interleukin-1 receptor type 2 prote Read more... |
Note | For research use only |
Application notes | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Expiration Date | 6 months from date of receipt. |
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. | |
The protein has a predicted molecular mass of 64.0 kDa after removal of the signal peptide. The apparent molecular mass of IL1R2-mFc is approximately 70-130 kDa due to glycosylation. | |
Mammalian |
ELISA, MS, SDS-PAGE, WB | |
Greater than 95% as determined by reducing SDS-PAGE. | |
Human cells |
> 95%, determined by SDS-PAGE | |
This protein contains the mouse IL1R2 (P27931) (Met1-Glu355) was expressed with a C-terminal polyhistidine tag. |
> 85%, determined by SDS-PAGE | |
This protein contains the mouse IL1R2 (P27931) (Met1-Glu355) was fused with the Fc region of human IgG1 at the C-terminus and expressed from HEK293 Cells. |