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Catalog Number | orb391946 |
---|---|
Category | Proteins |
Description | Recombinant mouse IL1B protein is produced by our E. coli. It is a single non-glycosylated polypeptide chain containing 152 amino acids with a molecular mass of 17400 Daltons. |
Tested applications | ELISA, MS, SDS-PAGE, WB |
Reactivity | Mouse |
Form/Appearance | Lyophilized from a 0.2 μM filtered solution of 50mM Tris-HCl, 50mM NaCl, pH 8.0. |
Purity | Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE. |
Target | IL1B |
Entrez | 16176 |
UniProt ID | P10749 |
Protein Sequence | VPIRQLHYRL RDEQQKSLVL SDPYELKALH LNGQNINQQV IFSMSFVQGE PSNDKIPVAL GLKGKNLYLS CVMKDGTPTL QLESVDPKQY PKKKMEKRFV FNKIEVKSKV EFESAEFPNW YISTSQAEHK PVFLGNNSGQ DIIDFTMESV SS |
Source | E. coli |
Endotoxins | Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test. |
Storage | Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months. |
Buffer/Preservatives | Lyophilized from a 0.2 μM filtered solution of 50mM Tris-HCl, 50mM NaCl, pH 8.0. |
Alternative names | IL1F2; Interleukin-1 beta; IL-1 beta; Catabolin Read more... |
Note | For research use only |
Application notes | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Expiration Date | 6 months from date of receipt. |
> 96% as determined by SDS-PAGE and HPLC. | |
17.5 kDa | |
E.Coli |
Unconjugated | |
95% | |
19.3 kDa | |
Mouse IL-1 beta, His Tag (orb762425) is expressed from E. coli cells. It contains AA Val 118 - Ser 269 (Accession # P10749-1). |
Greater than 90% as determined by SDS-PAGE. | |
21.2 kDa | |
E.coli |
> 95% as determined by SDS-PAGE | |
26 kDa |