Mouse IL10 protein

• Catalog Number: orb80068
• Category: Proteins
• Description: IL-10 Recombinant Mouse produced in E.coli is a single, non-glycosylated polypeptide chain containing 161 amino acids and having a molecular mass of 18785 Dalton. The IL10 Mouse is purified by proprietary chromatographic techniques.
• Tested applications: FA, HPLC, SDS-PAGE
• Purity: > 98.0% as determined by RP-HPLC and analysis by SDS-PAGE
• Conjugation: Unconjugated
• Target: mIL 10
• Solubility (25°C): It is recommended to reconstitute the lyophilized IL-10 Mouse Recombinant in sterile H₂O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
• Protein Sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Met-Ser-Arg-Gly-Gln.
• Biological Origin: E.coli
• Biological Activity: The ED50 as determined by the dose-dependant co-stimulation with IL-4 of mouse MC-9 cells was found to be 2ng/ml, corresponding to Specific Activity of x 100,000 IU/mg.
• Storage: Store at 4°C for up to two weeks. For long term storage, aliquot and store at -20°C, avoid freeze/thaw cycles.
• Buffer/Preservatives: The protein was lyophilized after extensive dialysis against PBS.
• Alternative names: B-TCGF protein, CSIF protein, TGIF protein, IL-10
• Note: For research use only
• Application notes: Experiment Notes: Protein quantitation was carried out by two independent methods1. UV spectroscopy at 280 nm using the absorbency value of 0.34 as the extinction coefficient for 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using calibrated solution of IL-10 as Reference Standard.
• Expiration Date: 6 months from date of receipt.

SDS-PAGE analysis of Mouse IL10 protein