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Catalog Number | orb391909 |
---|---|
Category | Proteins |
Description | Recombinant mouse IL10 protein is produced by our E. coli expression system and the target gene encoding Ser19-Ser178 is expressed. |
Tested applications | ELISA, MS, SDS-PAGE, WB |
Reactivity | Mouse |
Form/Appearance | Lyophilized from a 0.2 μM filtered solution of PBS, pH 7.4. |
Purity | Greater than 95% as determined by reducing SDS-PAGE. |
Target | IL10 |
Entrez | 16153 |
UniProt ID | P18893 |
Protein Sequence | MSRGQYSRED NNCTHFPVGQ SHMLLELRTA FSQVKTFFQT KDQLDNILLT DSLMQDFKGY LGCQALSEMI QFYLVEVMPQ AEKHGPEIKE HLNSLGEKLK TLRMRLRRCH RFLPCENKSK AVEQVKSDFN KLQDQGVYKA MNEFDIFINC IEAYMMIKMK S |
Source | E. coli |
Endotoxins | Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test. |
Storage | Lyophilized protein should be stored at -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at -20°C for 3 months. |
Buffer/Preservatives | Lyophilized from a 0.2 μM filtered solution of PBS, pH 7.4. |
Alternative names | Interleukin-10; IL-10; Cytokine synthesis inhibito Read more... |
Note | For research use only |
Application notes | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
Expiration Date | 6 months from date of receipt. |
> 97% as determined by SDS-PAGE and HPLC. | |
18.7 kDa | |
E.Coli |
Greater than 95% as determined by SDS-PAGE. | |
18.9 kDa | |
E.coli |
FA, HPLC, SDS-PAGE | |
Unconjugated | |
> 98.0% as determined by RP-HPLC and analysis by SDS-PAGE |
FA, HPLC, SDS-PAGE | |
Unconjugated | |
> 95.0% as determined by RP-HPLC and analysis by SDS-PAGE |
> 95% by SDS-PAGE. | |
KMP1717, Recombinant Mouse IL-10 Protein is produced by E. coli expression system. The target protein is expressed with sequence (Ser19-Ser178) of mouse IL-10 (Accession #P18893) fused with an initial Met at the N-terminus. |
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