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Catalog Number | orb1496304 |
---|---|
Category | Proteins |
Description | Mouse IL-7, His Tag is expressed from human 293 cells (HEK293). It contains AA Glu 26 - Ile 154 (Accession # Q544C8). |
Reactivity | Mouse |
Tag | C-10×His |
Form/Appearance | Powder |
Purity | 90% |
Conjugation | Unconjugated |
MW | 16.8 kDa |
Target | IL-7 |
UniProt ID | Q544C8 |
Source | Mouse IL-7, His Tag (orb1496304) is expressed from human 293 cells (HEK293). It contains AA Glu 26 - Ile 154 (Accession # Q544C8). |
Expression System | HEK293 |
Biological Origin | Mouse |
Biological Activity | Immobilized Mouse IL-7 Protein, His Tag at 2 μg/mL (100 μL/well) can bind Human IL-7 R alpha, Mouse IgG2a Fc Tag with a linear range of 0.2-2 ng/mL (QC tested). |
Expression Region | Glu 26 - Ile 154 |
Endotoxins | 0.1 EU per μg |
Storage | -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower. |
Buffer/Preservatives | PBS, pH7.4 |
Alternative names | IL7,Interleukin-7 Read more... |
Note | For research use only |
Application notes | This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 16.8 kDa. The protein migrates as 20 kDa and 26-30 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Expiration Date | 6 months from date of receipt. |
Immobilized Mouse IL-7 Protein, His Tag (Cat. No. orb1496304) at 2 μg/mL (100 μL/well) can bind Human IL-7 R alpha, Mouse IgG2a Fc Tag (Cat. No. orb402998) with a linear range of 0.2-2 ng/mL (QC tested).
Greater than 95% as determined by SDS-PAGE. | |
15.9 kDa | |
Mammalian cell |
> 96% as determined by SDS-PAGE and HPLC. | |
14.9 kDa | |
E.Coli |
Unconjugated | |
90% | |
52.0 kDa | |
Human IL-7 R alpha, Mouse IgG2a Fc Tag (orb402998) is expressed from human 293 cells (HEK293). It contains AA Glu 21 - Gly 236 (Accession # P16871-1 (I66T,V138I)). |