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Catalog Number | orb1149287 |
---|---|
Category | Proteins |
Description | Mouse IL-4, His Tag is expressed from human 293 cells (HEK293). It contains AA His 21 - Ser 140 (Accession # P07750-1). |
Reactivity | Mouse |
Tag | C-10×His |
Form/Appearance | Powder |
Purity | 95% |
Conjugation | Unconjugated |
MW | 15.4 kDa |
Target | IL-4 |
UniProt ID | P07750 |
Source | Mouse IL-4, His Tag (orb1149287) is expressed from human 293 cells (HEK293). It contains AA His 21 - Ser 140 (Accession # P07750-1). |
Expression System | HEK293 |
Biological Origin | Mouse |
Biological Activity | Immobilized Mouse IL-4, His Tag at 5 μg/mL (100 μL/well) can bind Mouse IL-4 R alpha, Fc Tag with a linear range of 0.2-16 ng/mL (QC tested). |
Expression Region | His 21 - Ser 140 |
Endotoxins | 1.0 EU per μg |
NCBI | NP_067258.1 |
Storage | -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower. |
Buffer/Preservatives | PBS, pH7.4 |
Alternative names | IL4,BCGF1,BSF1 Read more... |
Note | For research use only |
Application notes | This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 15.4 kDa. The protein migrates as 17-20 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Expiration Date | 6 months from date of receipt. |
Immobilized Mouse IL-4, His Tag (Cat. No. orb1149287) at 5 μg/mL (100 μL/well) can bind Mouse IL-4 R alpha, Fc Tag (Cat. No. orb334907) with a linear range of 0.2-16 ng/mL (QC tested).
Unconjugated | |
95% | |
26.3 kDa | |
Mouse IL-4 R alpha, His Tag (orb334908) is expressed from human 293 cells (HEK293). It contains AA Ile 26 - Arg 233 (Accession # NP_001008700). |
> 90%, determined by SDS-PAGE | |
This protein contains the mouse IL4 (NP_067258.1) (His23-Ser140) was expressed with a N-terminal Met. |
> 95%, determined by SDS-PAGE | |
This protein contains the mouse IL4 (P07750) (Met 1-Ser140, Q136D, Y139D) was expressed with a polyhistidine tag at the C-terminus and expressed from Baculovirus-Insect Cells. |
98.00% | |
14.9 kDa (predicted); 19 kDa (reducing condition, due to glycosylation) |