You have no items in your shopping cart.
You have no items in your shopping cart.
Catalog Number | orb653661 |
---|---|
Category | Proteins |
Description | Mouse IL-17F, His Tag is expressed from human 293 cells (HEK293). It contains AA Arg 29 - Ala 161 (Accession # Q7TNI7-1). |
Reactivity | Mouse |
Tag | N-10×His |
Form/Appearance | Powder |
Purity | 90% |
Conjugation | Unconjugated |
MW | 16.8 kDa |
Target | IL-17F |
UniProt ID | Q7TNI7 |
Source | Mouse IL-17F, His Tag (orb653661) is expressed from human 293 cells (HEK293). It contains AA Arg 29 - Ala 161 (Accession # Q7TNI7-1). |
Expression System | HEK293 |
Biological Origin | Mouse |
Biological Activity | Human IL-17 RA, Fc Tag captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind Mouse IL-17F, His Tag with an affinity constant of 2.99 μM as determined in a SPR assay (Biacore 8K) (Routinely tested). |
Expression Region | Arg 29 - Ala 161 |
Endotoxins | 0.1 EU per μg |
Storage | -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower. |
Buffer/Preservatives | PBS, pH7.4 |
Alternative names | IL-17F,Interleukin-17F,Cytokine ML-1 Read more... |
Note | For research use only |
Application notes | This protein carries a polyhistidine tag at the N-terminus. The protein has a calculated MW of 16.8 kDa. The protein migrates as 24-25 kDa and 27-28 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Expiration Date | 6 months from date of receipt. |
Human IL-17 RA, Fc Tag captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind Mouse IL-17F, His Tag (Cat. No. orb653661) with an affinity constant of 2.99 μM as determined in a SPR assay (Biacore 8K) (Routinely tested).
98.00% | |
17.8 kDa (predicted). Due to glycosylation, the protein migrates to 30-35 kDa based on Tris-Bis PAGE result. |
> 95% | |
17.8 kDa (predicted). Due to glycosylation, the protein migrates to 30-35 kDa based on Tris-Bis PAGE result. |
> 95% by SDS-PAGE. | |
Recombinant Mouse IL-17F Protein is produced by HEK293 Cells expression system. The target protein is expressed with sequence (Met1-Ala161) of mouse IL-17F (Accession #NP_665855.2) fused with a 6��His Tag at the C-terminus. |