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Catalog Number | orb1173321 |
---|---|
Category | Assays and Kits |
Description | Mouse IL-17 ELISA Kit employs a two-site sandwich ELISA to quantitate Mouse IL-17. |
Tested applications | ELISA |
Reactivity | Mouse |
Tag | IL17 |
Entrez | 16171 |
UniProt ID | Q62386 |
Storage | The unopened kit should be stored at 2 - 8°C. After opening, please store refer to protocols. |
Alternative names | IL 17, IL17A, CTLA8, IL-17, IL-17A, IL17, cytotoxi Read more... |
Note | For research use only |
Application notes | Mouse IL-17 ELISA Kit employs a two-site sandwich ELISA to quantitate IL-17 in samples. An antibody specific for IL-17 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-17 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL-17 is added to the wells. After washing, proprietary Streptavidin-HRP conjugates is added to the wells. Following a wash to remove any unbound streptavidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL-17 bound in the initial step. The color development is stopped and the intensity of the color is measured. |
Sample Types | Cell culture supernatants#Serum#Plasma#Other biological fluids |
Expiration Date | Please enquire. |
Mouse | |
78 pg/ml - 5000 pg/ml | |
19.5 pg/ml |
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