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Catalog Number | orb257586 |
---|---|
Category | Proteins |
Description | Mouse IL-13RA1, Fc Tag is expressed from human 293 cells (HEK293). It contains AA Ala 26 - Thr 340 (Accession # NP_598751). |
Reactivity | Mouse |
Tag | C-Fc |
Form/Appearance | Powder |
Purity | 95% |
Conjugation | Unconjugated |
MW | 62.7 kDa |
Target | IL-13 R alpha 1 |
UniProt ID | O09030 |
Source | Mouse IL-13RA1, Fc Tag (orb257586) is expressed from human 293 cells (HEK293). It contains AA Ala 26 - Thr 340 (Accession # NP_598751). |
Expression System | HEK293 |
Biological Origin | Mouse |
Biological Activity | Loaded Mouse IL-13RA1, Fc Tag on Protein A Biosensor, can bind Human IL-13, His Tag with an affinity constant of 22.5 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested). |
Expression Region | Ala 26 - Thr 340 |
Endotoxins | 1.0 EU per μg |
NCBI | NP_598751 |
Storage | -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower. |
Buffer/Preservatives | Tris with Glycine, Arginine and NaCl, pH7.5 |
Alternative names | IL13RA1,CD213A1,IL-13Ra,NR4,RP13-128O4.2,IL13Rα1 Read more... |
Note | For research use only |
Application notes | This protein carries a human IgG1 Fc tag at the C-terminus. The protein has a calculated MW of 62.7 kDa. The protein migrates as 67-100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Expiration Date | 6 months from date of receipt. |
SDS-PAGE analysis of Mouse IL-13 R alpha 1 protein
Loaded Mouse IL-13RA1, Fc Tag (Cat. No. orb257586) on Protein A Biosensor, can bind Human IL-13, His Tag (Cat. No. orb612135) with an affinity constant of 22.5 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Unconjugated | |
95% | |
37.9 kDa | |
Mouse IL-13 R alpha 1, His Tag (orb257587) is expressed from human 293 cells (HEK293). It contains AA Ala 26 - Thr 340 (Accession # NP_598751). |
Greater than 95% as determined by reducing SDS-PAGE | |
20 KDa (reducing condition) |
> 95% as determined by Bis-Tris PAGE > 95% as determined by HPLC | |
37.55 kDa (predicted). Due to glycosylation, the protein migrates to 45-60 kDa based on Tris-Bis PAGE result. |