Mouse IgM (mu chain) Antibody

• Catalog Number: orb347418
• Category: Antibodies
• Description: Mouse IgM (mu chain) antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: DOT, ELISA, IHC, WB
• Reactivity: Mouse
• Isotype: IgG
• Immunogen: Mouse IgM whole molecule
• Concentration: 2.19 mg/mL
• Dilution range: ELISA: 1:20,400 - 1:40,400, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
• Form/Appearance: Liquid (sterile filtered)
• Purity: Anti-Mouse IgM (mu chain) was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgM coupled to agarose followed by solid phase adsorption(s). Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse IgM and Mouse Serum. No reaction was observed against other mouse heavy or light chain proteins.
• Conjugation: Unconjugated
• Storage: Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide. 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Alternative names: goat anti-Mouse IgM (mu chain) Antibody, Gt-a-Mous
• Note: For research use only
• Application notes: Anti-Mouse IgM (mu chain) has been tested by ELISA, dot blot, and western blot and is suitable for immunoblotting (western or dot blot), ELISA, and immunohistochemistry assays requiring lot-to-lot consistency.
• Expiration Date: 12 months from date of receipt.

Dot Blot of Mouse IgM (mu chain) (GOAT) Antibody. Lane 1A: Mouse IgM (100 ng). Lane 1B: Mouse IgG (100 ng). Lanes 2-5: serial 3-fold dilution. Primary Antibody: Mouse IgM (mu chain) Antibody at 1 µg/ml at RT for 60 minutes. Secondary Antibody: Donkey Anti-Goat IgG Antibody HRP Conj'd (p/n orb347032) at 1;40000 dilution of 1 mg/ml at RT for 30 minutes. Block: orb348637 at RT for 30 min.