Mouse IgM Kappa isotype Control Antibody

• Catalog Number: orb343770
• Category: Proteins
• Description: Mouse IgM Kappa isotype Control Antibody
• Tested applications: ELISA, FC, FLISA, IF, SDS-PAGE, WB
• Isotype: IgM
• Concentration: 1.0mg/mL
• Dilution range: ELISA: User Optimized, FLISA: User Optimized, FC: 1:1000-1:5000, IF: User Optimized, WB: User Optimized
• Form/Appearance: Liquid (sterile filtered)
• Purity: Mouse IgM Kappa isotype control was prepared from concentrated cell culture supernatant by a multi-step process which includes selective precipitation and tandem molecular sieve chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse IgM mu and anti-Mouse serum. Isotyping assay resulted non-reactive with antisera to mouse IgG1, IgG2a, IgG2b, Ig3, IgA. Light chain composition has been confirmed by SDS-PAGE.
• Conjugation: Unconjugated
• Source: Mouse
• Biological Origin: Mouse
• Storage: Store vial at 4° C prior to opening. Mouse IgM Kappa (κ) isotype control is stable 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
• Buffer/Preservatives: 0.1% (w/v) Sodium Azide. 0.1 M Tris Chloride, 0.5 M Sodium Chloride, pH 8.0
• Alternative names: Mouse immunoglobulin M kappa, Mouse IgM isotype co
• Note: For research use only
• Application notes: Mouse IgM kappa isotype control has been tested in ELISA and can be utilized as a control or standard reagent in Flow cytometry, Western Blotting, and ELISA experiments where determination of sample isotype is important.
• Expiration Date: 12 months from date of receipt.

SDS-PAGE of Mouse IgG Whole Molecule Rhodamine Conjugated (p/n orb346272). Lane 1: 5 µL Opal Prestained Marker. Lane 2: Reduced Mouse IgG Whole Molecule Rhodamine Conjugated (p/n orb346272). Lane 3: Reduced Mouse F(c) Fragment (p/n orb346280). Lane 4: Reduced Mouse F(ab) Fragment (p/n orb346282). Lane 5: Mouse IgM Kappa Myeloma Protein (p/n orb346283). Load: 1 µg per lane. Predicted/Observed size: IgG at 50 and 25 kDa; F(c) at 25 kDa; F(ab) at 25 kDa; IgM K at 70 and 23 kDa. Observed F(c) Fragment migrates slightly higher.