Mouse IgM Isotype Control antibody

• Catalog Number: orb43568
• Category: Antibodies
• Description: Mouse IgM Isotype Control
• Clonality: Monoclonal
• Clone Number: PFR-03
• Tested applications: ELISA, FC, ICC, IHC-Fr, IHC-P, IP, WB
• Isotype: Mouse IgM
• Concentration: 1 mg/ml
• Dilution range: Negative control: The reagent is intended as an isotype control to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 μg/ml of incubation mixture), change the conditions of your experiment to reduce the background.
• Purity: Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods).
• Conjugation: Unconjugated
• RRID: AB_10990517
• Storage: Store at 2-8°C. Do not freeze.
• Buffer/Preservatives: Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide
• Note: For research use only
• Application notes: Negative control: The reagent is intended as an isotype control to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 μg/ml of incubation mixture), change the conditions of your experiment to reduce the background.
• Expiration Date: 12 months from date of receipt.

Flow cytometry analysis: Example of nonspecific mouse IgM (PFR-03) PE signal on human peripheral blood; surface staining, 3 µg/ml.