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Catalog Number | orb43559 |
---|---|
Category | Antibodies |
Description | Mouse IgG3 Isotype Control |
Clonality | Monoclonal |
Clone Number | PPV-07 |
Tested applications | ELISA, FC, ICC, IHC-Fr, IHC-P, IP, WB |
Isotype | Mouse IgG3 |
Concentration | 1 mg/ml |
Dilution range | Negative control: The reagent is intended as an isotype control to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 μg/ml of incubation mixture), change the conditions of your experiment to reduce the background. |
Purity | Purified by protein-A affinity chromatography. |
Conjugation | Unconjugated |
RRID | AB_10994887 |
Storage | Store at 2-8°C. Do not freeze. |
Buffer/Preservatives | HEPES buffered saline (HBS), pH 7.0, 15 mM sodium azide |
Note | For research use only |
Application notes | Negative control: The reagent is intended as an isotype control to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 μg/ml of incubation mixture), change the conditions of your experiment to reduce the background. |
Expiration Date | 12 months from date of receipt. |
Flow cytometry surface nonspecific staining pattern of human peripheral whole blood stained using mouse IgG3 Isotype control (PPV-07) purified antibody (concentration in sample 9 μg/ml).
ELISA, FC, FLISA, IF, SDS-PAGE, WB | |
Unconjugated | |
Mouse IgG3 Isotype control has been prepared from concentrated cell culture supernatant by immunoaffinity chromatography using protein A. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse IgG3 and anti-Mouse serum. Isotyping assay resulted non-reactive with antisera to mouse IgG1, IgG2a, IgG2b, IgA, IgM. Light chain composition has been confirmed by SDS-PAGE. | |
Mouse |
ELISA, FC, FLISA, IF, SDS-PAGE, WB | |
Unconjugated | |
Mouse Isotype control has been prepared from concentrated cell culture supernatant by immunoaffinity chromatography using protein A. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse IgG3 and anti-Mouse serum. Isotyping assay resulted non-reactive with antisera to mouse IgG1, IgG2a, IgG2b, IgA, IgM. Light chain composition has been confirmed by SDS-PAGE. | |
Mouse |
ELISA, FC, FLISA, IF, SDS-PAGE, WB | |
Unconjugated | |
Mouse Isotype control has been prepared from concentrated cell culture supernatant by immunoaffinity chromatography using protein A. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse IgG3 and anti-Mouse serum. Isotyping assay resulted non-reactive with antisera to mouse IgG1, IgG2a, IgG2b, IgA, IgM. Confirmed by SDS-PAGE. | |
Mouse |
DOT, ELISA, FC, FLISA, IF, WB | |
FITC | |
This product has been prepared from serum-free tissue culture supernate by protein A chromatography. Typically less than 1% cross reactivity against other mouse heavy or light chain specific antibodies was detected by ELISA. | |
Mouse |
Control | |
Recombinant | |
Unconjugated |
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