Mouse IgG3 isotype Control Antibody

• Catalog Number: orb343779
• Category: Proteins
• Description: Mouse IgG3 isotype Control Antibody
• Tested applications: ELISA, FC, FLISA, IF, SDS-PAGE, WB
• Isotype: IgG3
• Concentration: 1.0 mg/mL
• Dilution range: ELISA: User Optimized, FLISA: User Optimized, FC: 1:1000-1:5000, IF: User Optimized, WB: User Optimized
• Form/Appearance: Liquid (sterile filtered)
• Purity: Mouse Isotype control has been prepared from concentrated cell culture supernatant by immunoaffinity chromatography using protein A. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse IgG3 and anti-Mouse serum. Isotyping assay resulted non-reactive with antisera to mouse IgG1, IgG2a, IgG2b, IgA, IgM. Confirmed by SDS-PAGE.
• Conjugation: Unconjugated
• Source: Mouse
• Biological Origin: Mouse
• Storage: Store vial at 4° C prior to opening. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: MOUSE IgG3 subclass isotype control, MOUSE IgG3 is
• Note: For research use only
• Application notes: Mouse IgG3 isotype control has been tested by SDS-Page and ELISA and can be utilized as a control or standard reagent in Flow cytometry, Western Blotting, and ELISA experiments where determination of sample isotype is important.
• Expiration Date: 12 months from date of receipt.

SDS-PAGE of Mouse IgG3. Lane 1: Non-Reduced Human IgG. Lane 2: [5 µL] Opal Pre-stained Marker. Lane 3: Reduced Mouse IgG3. Load: 1 µg per lane. Predicted/Observed size: Non-reduced at 180-245 kDa, Reduced at 55, 25 kDa.