Mouse IgG2b isotype control Phycoerythrin Antibody

• Catalog Number: orb343794
• Category: Proteins
• Description: Mouse IgG2b isotype control Phycoerythrin Antibody
• Tested applications: DOT, FC, FLISA, IF
• Isotype: IgG2b
• Concentration: 1 mg/ml
• Dilution range: FLISA: User Optimized, FC: User Optimized, IF: 1:1000-1:5000
• Form/Appearance: Lyophilized
• Purity: This product was prepared from ascitic fluid by affinity chromatography. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse IgG2b and anti-Mouse serum. Isotyping assay resulted the material is non-reactive with antisera to mouse IgG1, IgG2a, IgG3, IgM, and IgA. Phycoerythrin Conjugated Mouse IgG2b was proven by Dot Blot.
• Conjugation: RPE
• Source: Mouse
• Biological Origin: Mouse
• Storage: Store vial at 4° C prior to restoration. Restore with deionized water (or equivalent). This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Centrifuge product if not completely clear after standing at room temperature. Do not freeze after reconstitution. Store reagent in the dark. Use subdued lighting during handling and incubation of cells prior to analysis.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: Phycoerythrin conjugation Mouse IgG2b isotype cont
• Note: For research use only
• Application notes: Mouse IgG2b isotype control has been tested in dot blot and can be utilized as a control or standard reagent in Flow cytometry, Western Blotting, and ELISA experiments where determination of sample isotype is important.
• Expiration Date: 12 months from date of receipt.

Dot Blot of Mouse IgG2b Isotype Control Phycoerythrin Conjugated. Antigen: none. Load: Lane 1 - 50 ng Lane 2 - 16.67 ng Lane 3 - 5.56 ng Lane 4 - 1.85 ng Lane 5 - 0.62 ng. Mouse IgG2b Isotype Control Phycoerythrin Conjugated blotted directly to membrane at above dilutions. Block: orb348637 for 60 min at RT.