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Catalog Number | orb343777 |
---|---|
Category | Proteins |
Description | Mouse IgG2a isotype Control Antibody |
Tested applications | ELISA, FC, FLISA, IF, SDS-PAGE, WB |
Isotype | IgG2a |
Concentration | 1.0 mg/mL |
Dilution range | ELISA: User Optimized, FLISA: User Optimized, FC: 1:1000-1:5000, IF: User Optimized, WB: User Optimized |
Form/Appearance | Liquid |
Purity | Mouse Isotype control has been prepared from concentrated cell culture supernatant by immunoaffinity chromatography using protein A. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse IgG2a and anti-Mouse Serum. Isotyping assay resulted the material is non-reactive with antisera to mouse IgG1, IgG2b, IgG3, IgM, and IgA. Confirmed by SDS-PAGE. |
Conjugation | Unconjugated |
Source | Mouse |
Biological Origin | Mouse |
Storage | Store vial at 4° C prior to opening. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
Alternative names | MOUSE IgG2a isotype Control Read more... |
Note | For research use only |
Application notes | Mouse IgG2a isotype control has been tested by SDS-Page and can be utilized as a control or standard reagent in Flow cytometry, Western Blotting, and ELISA experiments where determination of sample isotype is important. |
Expiration Date | 12 months from date of receipt. |
SDS-Page Results of Mouse IgG2a Isotype Control. Lane 1: Mouse IgG2a Isotype Control Reduced [1.0 µg]. Lane 2: Opal Prestained Molecular Weight Marker. Lane 3: Mouse IgG2a Isotype Control Non-Reduced [1.0 µg]. 4-20% gel. Coomassie stained.
ELISA, FACS, IF, IHC-P, WB | |
Mouse | |
Monoclonal | |
Unconjugated |
ELISA, FACS, IF, IHC-P, WB | |
Mouse | |
Monoclonal | |
Unconjugated |
ELISA, FACS, IF, IHC-P, WB | |
Mouse | |
Monoclonal | |
Unconjugated |
FC, IF | |
Mouse | |
Monoclonal | |
Unconjugated |
FC, IF | |
Mouse | |
Monoclonal | |
PE/Cy5 |
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