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Catalog Number | orb343762 |
---|---|
Category | Proteins |
Description | Mouse IgG1 Lambda isotype Control Antibody |
Tested applications | ELISA, FC, FLISA, IF, SDS-PAGE, WB |
Isotype | IgG1 |
Concentration | 1.0 mg/mL |
Dilution range | ELISA: User Optimized, FLISA: User Optimized, FC: 1:1000-1:5000, IF: User Optimized, WB: User Optimized |
Form/Appearance | Liquid (sterile filtered) |
Purity | Mouse Isotype control has been prepared from concentrated cell culture supernatant by immunoaffinity chromatography using protein A. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse IgG and anti-Mouse serum. Isotyping assay resulted non-reactive with antisera to mouse IgG2a, IgG2b, IgG3, IgA, IgM. Light chain composition has been confirmed by SDS-PAGE. |
Conjugation | Unconjugated |
Source | Mouse |
Biological Origin | Mouse |
Storage | Store vial at 4° C prior to opening. Mouse IgG1 Lambda isotype control is stable 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. |
Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
Alternative names | Mouse immunoglobulin gamma lambda, Mouse IgG1 isot Read more... |
Note | For research use only |
Application notes | Mouse IgG1 lambda isotype control has been tested in SDS-Page and ELISA and can be utilized as a control or standard reagent in Flow cytometry, Western Blotting, and ELISA experiments where determination of sample isotype is important. |
Expiration Date | 12 months from date of receipt. |
SDS-PAGE of Mouse IgG1 Lambda Isotype Control. Lane 1: Mouse IgG1 Lambda Isotype Control, Reduced. M: Opal Pre-stained Ladder. Lane 2: Mouse IgG1 Lambda Isotype Control, Non-reduced. Load: 1.0 µg per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.
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