Mouse IgG1 Isotype Control antibody

• Catalog Number: orb43526
• Category: Antibodies
• Description: Mouse IgG1 Isotype Control
• Clonality: Monoclonal
• Clone Number: MOPC-21
• Tested applications: ELISA, FA, FC, ICC, IHC-Fr, IHC-P, IP, WB
• Isotype: Mouse IgG1 kappa
• Concentration: 1 mg/ml
• Dilution range: Negative control: The reagent is intended as an isotype control to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 μg/ml of incubation mixture), change the conditions of your experiment to reduce the background.
• Purity: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated
• RRID: AB_10999249
• Storage: Store at 2-8°C. Do not freeze.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4
• Note: For research use only
• Application notes: Low Endotoxin, Azide free format. Product does not contain preservatives and is intended primarily for functional applications. Preservative containing buffers are available for IHC applications
• Expiration Date: 12 months from date of receipt.

Flow cytometry surface nonspecific staining pattern of human peripheral whole blood stained using mouse IgG1 Isotype control (MOPC-21) purified antibody (low endotoxin, concentration in sample 9 μg/ml).