Mouse IgG1 isotype Control Antibody

• Catalog Number: orb343776
• Category: Proteins
• Description: Mouse IgG1 isotype Control Antibody
• Tested applications: ELISA, FC, FLISA, IF, SDS-PAGE, WB
• Isotype: IgG1
• Concentration: 1.0 mg/mL
• Dilution range: ELISA: User Optimized, FLISA: User Optimized, FC: 1:1000-1:5000, IF: User Optimized, WB: User Optimized
• Form/Appearance: Liquid (sterile filtered)
• Purity: Mouse IgG1 Isotype control has been prepared from concentrated cell culture supernatant by immunoaffinity chromatography using protein A. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse IgG1 and anti-Mouse Serum. Isotyping assay resulted the material is non-reactive with antisera to mouse IgG2a, IgG2b, IgG3, IgM, and IgA. SDS-PAGE shows a band at 60 kDa corresponding to alpha heavy chain.
• Conjugation: Unconjugated
• Source: Mouse
• Biological Origin: Mouse
• Storage: Store vial at 4° C prior to opening. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: Mouse IgG1 isotype, Mouse IgG1 subclass isotype, M
• Note: For research use only
• Application notes: Mouse IgG1 isotype control has been tested by ELISA and SDS-Page and can be utilized as a control or standard reagent in Flow cytometry, Western Blotting, and ELISA experiments where determination of sample isotype is important.
• Expiration Date: 12 months from date of receipt.

SDS-PAGE of Mouse IgG1 Isotype Control. Lane 1: Mouse IgG1 Isotype Control, Non-reduced. M: Opal Pre-stained Ladder. Lane 2: Mouse IgG1 Isotype Control, Reduced. Load: 1.0 µg per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.