Mouse IgG1 Antibody / Isotype Control for Mouse

• Catalog Number: orb2636478
• Category: Antibodies
• Description: To serve as a true negative control for various procedures, this mouse monoclonal IgG should be used under the identical conditions and at the same dilution as the rabbit monoclonal antibody being used for a positive reaction.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: ICG1/1331
• Tested applications: FACS, IF, IHC-P, WB
• Reactivity: Human
• Isotype: Mouse IgG1, kappa
• Immunogen: Recombinant full-length human IG1R protein was used as the immunogen for the mouse IgG1 antibody.
• Dilution range: Flow cytometry: application dependent ,Immunofluorescence: application dependent,Immunohistochemistry (FFPE): application dependent,Western blot: application dependent
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This mouse IgG1 antibody is available for research use only.
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Note: For research use only
• Application notes: Optimal dilution of the mouse IgG1 antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

IHC staining of FFPE human heart tissue with mouse IgG1 antibody (clone ICG1/ 1331). HIER: boil tissue sections in pH9 10 mM Tris with 1 mM EDTA for 20 min and allow to cool before testing. No staining was observed.

Immunofluorescent staining of PFA-fixed human Raji cells with mouse IgG1 antibody (clone ICG1/ 1331, green) and Reddot nuclear stain (red). No IgG1 staining was observed.

SDS-PAGE analysis of purified, BSA-free mouse IgG1 antibody (clone ICG1/ 1331) as confirmation of integrity and purity.