Mouse IgG IgA IgM (H&L) Antibody Fluorescein Conjugated

• Catalog Number: orb347378
• Category: Antibodies
• Description: Mouse IgG IgA IgM (H&L) antibody (FITC)
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: DOT, FC, FLISA, IF
• Reactivity: Mouse
• Isotype: IgG
• Immunogen: Mouse IgG IgA and IgM whole molecule
• Concentration: 2.0 mg/mL
• Dilution range: FLISA: 1:10,000 - 1:50,000, FC: 1:500 - 1:2,500, IF: 1:1,000 - 1:5,000
• Form/Appearance: Lyophilized
• Purity: This product was prepared from polyspecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Goat Serum, Mouse IgG, Mouse IgA and Mouse IgM. This reagent is suitable for the detection of all mouse isotypes and chain combinations.
• Conjugation: FITC
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: Goat anti-mouse IgG IgA IgM antibody fluorescein c
• Note: For research use only
• Application notes: Anti-Mouse IgG IgA IgM Fluorescein Antibody has been tested by dot blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
• Expiration Date: 12 months from date of receipt.

Dot Blot for Mouse IgG IgA IgM (H&L) Antibody Fluorescein Conjugated. Lane 1A: 100 ng Ms IgG. Lane 1B: 100 ng Ms IgA. Lane 1C: 100 ng Ms IgM. Lanes 2-5: serial dilution 3 fold of respective Antigen. Primary Antibody: none. Secondary Antibody: Goat Anti-Mouse IgG IgA IgM (H&L) Antibody Fluorescein Conjugated at 1:1000 dilution at RT for 1 hour. Block: orb348637 at RT for 30 minutes.