Mouse IgG (H&L) Secondary Antibody Alkaline Phosphatase Conjugated

• Catalog Number: orb347558
• Category: Antibodies
• Description: MOUSE IgG (H&L) antibody (Alkaline Phosphatase)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: DOT, ELISA, IHC, WB
• Reactivity: Mouse
• Isotype: IgG
• Immunogen: Mouse IgG whole molecule
• Concentration: 1.0 mg/mL
• Dilution range: ELISA: 1:2,000 - 1:10,000, IHC: 1:200 - 1:1,000, WB: 1:500 - 1:2,500
• Form/Appearance: Liquid (sterile filtered)
• Purity: Secondary antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Rabbit Serum, Mouse IgG and Mouse Serum.
• Conjugation: AP
• Storage: Store secondary antibody at 4° C before opening. DO NOT FREEZE. Mouse conjugated antibody is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: rabbit anti-Mouse IgG Antibody alkaline phosphatas
• Note: For research use only
• Application notes: Anti-Mouse IgG Alkaline Phosphatase antibody has been tested by ELISA, dot blot, and western blot and is ideal for western blotting, Immunohistochemistry and ELISA as well as other antibody detection methods.
• Expiration Date: 12 months from date of receipt.

Western Blot of Rabbit anti-Mouse IgG Antibody Alkaline Phosphatase Conjugated. Lane 1: Mouse IgG. Load: 100 ng per lane. Secondary antibody: MOUSE IgG (H&L) Antibody Alkaline Phosphatase Conjugated at 1:1000 for 60 min at RT. Block: orb348637 for 30 min at RT. Predicted/Observed size: 55 and 28 kDa, 55 and 28 kDa.