Mouse IgG (H&L) Secondary Antibody Fluorescein Conjugated

• Catalog Number: orb347530
• Category: Antibodies
• Description: Mouse IgG (H&L) antibody (FITC)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: DOT, FLISA, IF, WB
• Reactivity: Mouse
• Isotype: IgG
• Immunogen: Mouse IgG whole molecule
• Concentration: 2.0 mg/mL
• Dilution range: FLISA: 1:10,000 - 1:50,000, IF: 1:1,000 - 1:5,000
• Form/Appearance: Lyophilized
• Purity: Secondary Antibody Conjugate was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Rabbit Serum, Mouse IgG and Mouse Serum.
• Conjugation: FITC
• Biological Activity: 2.7
• Storage: Store Conjugated Second Antibody at 4° C prior to restoration. For extended storage aliquot antibody and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: Rabbit Anti-Mouse IgG Antibody fluorescein Conjuga
• Note: For research use only
• Application notes: Anti-Mouse IgG Fluorescein Antibody has been tested by dot blot and western blot and is designed for flow cytometry, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
• Expiration Date: 12 months from date of receipt.

Western blot of Fluorescein conjugated Rabbit Anti-Mouse IgG secondary antibody. Lane 1: Mouse IgG. Lane 2: None. Load: 50 ng per lane. Primary antibody: None. Secondary antibody: Fluorescein rabbit secondary antibody at 1:1000 for 60 min at RT. Blocking: orb348637 for 30 min at RT. Predicted/Observed size: 25 & 55 kDa, 25 & 55 kDa for Mouse IgG. Other band(s): None.