Mouse IgG (H&L) Secondary Antibody Alkaline Phosphatase Conjugated

• Catalog Number: orb347435
• Category: Antibodies
• Description: MOUSE IgG (H&L) antibody (Alkaline Phosphatase)
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: DOT, ELISA, IHC, WB
• Reactivity: Mouse
• Isotype: IgG
• Immunogen: Mouse IgG whole molecule
• Concentration: 1.0 mg/mL
• Dilution range: ELISA: 1:15,000, IHC: 1:200 - 1:1,000, WB: 1:1,000 - 1:4,000
• Form/Appearance: Liquid (sterile filtered)
• Purity: Anti-Mouse Conjugated Secondary Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Goat Serum, Mouse IgG and Mouse Serum.
• Conjugation: AP
• Storage: Store secondary antibody conjugate at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
• Buffer/Preservatives: 0.1% (w/v) Sodium Azide
• Alternative names: Goat Anti-Mouse IgG Antibody alkaline phosphatase
• Note: For research use only
• Application notes: Mouse secondary antibody conjugated to Alkaline Phosphatase is available in a variety of formats. Anti-Mouse IgG Alkaline Phosphatase Antibody has been tested by ELISA, dot blot, and western blot and is suitable for ELISA, Immunohistochemistry, Western Blotting as well as other Alkaline Phosphatase antibody based assays.
• Expiration Date: 12 months from date of receipt.

Western Blot of Alkaline Phosphatase Conjugated Goat Anti-Mouse IgG Antibody Lane 1: Mouse IgG Load: 100 ng per lane Secondary antibody: Alkaline Phosphatase Conjugated Goat Anti-Mouse IgG Antibody at 1:1000 for 60 min at RT Block: orb348637 for 30 min RT Predicted/Observed size: 55 and 28 kDa, 55 and 28 kDa