Mouse IgG (H&L) Secondary Antibody Alkaline Phosphatase Conjugated Pre-Adsorbed

• Catalog Number: orb347394
• Category: Antibodies
• Description: Mouse IgG (H&L) antibody (Alkaline Phosphatase)
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: DOT, ELISA, IHC, WB
• Reactivity: Mouse
• Isotype: IgG
• Immunogen: Mouse IgG whole molecule
• Concentration: 1.0 mg/ml
• Dilution range: ELISA: 1:2,000 - 1:10,000, IHC: 1:200 - 1:1,000, WB: 1:500 - 1:2,500
• Form/Appearance: Liquid (sterile filtered)
• Purity: Mouse Secondary Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Goat Serum, Mouse IgG and Mouse Serum. No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Rabbit, Rat and Sheep Serum Proteins.
• Conjugation: AP
• Storage: Store secondary antibody at 4° C before opening. DO NOT FREEZE. Conjugated antibody is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
• Buffer/Preservatives: 0.09% (w/v) Sodium Azide
• Alternative names: Goat Anti-Mouse IgG Antibody alkaline phosphatase
• Note: For research use only
• Application notes: Anti-Mouse IgG Alkaline Phosphatase Antibody has been tested by ELISA an dot blot and is ideal for western blot, Immunohistochemistry and ELISA as well as other antibody detection methods; and are available in a variety of formats and conjugate types.
• Expiration Date: 12 months from date of receipt.

Dot Blot of Goat anti-Mouse IgG antibody Alkaline Phosphatase Conjugated (Min X Bv Ch Gt GP Ham Hs Hu Rb Rt & Sh Serum Proteins. Antigen: Mouse IgG. Load: 200 ng, 66.7 ng, 22.2 ng, 7.41 ng, or 2.47 ng as indicated. Primary antibody: Anti-Mouse IgG antibody Alkaline Phosphatase Conjugated at 1:1000 for 60 min at 4°C. Secondary antibody: N/A. Block: Blocking Buffer for Fluorescent Western Blotting (orb348637) for 60 min at RT.